Chemistry, Biotechnology and Food Science

Genomic Platform

Ingolf F. Nes

Laboratory of Microbial Gene Technology (LMG)

The facilities are part of the infrastructure of the department and located at LMG and are freely available for all researchers at the Ås campus.

The facilities of the genomic platform are composed of the following instruments:

A. DNA sequencer
(Responsible: Cand. scient Linda Godager)
Applied Biosystem: ABI PrismTM 3100 Genetic Analyzer

B. DNA/RNA analysis equipments
(Responsible: Cand scient Linda Godager)
1. Nano-Drop ND-1000 Spectrophotometer
2. Agilent 2100 bioanalyzer
3. Quantitative PCR machine: 7700 Sequence Detector

C. DNA microarray
(Responsible: Stud. Heidi Vebø and Dr. scient Ågot Aakra).
1. TECAN LS Reload Scanner
2. Packard ScanArray Express
3. Affimetrix DNA array facilities are available at Cigene at UMB

D. TECAN Washing/Hybridization Station HS 400 Pro
(Responsible: Stud. Heidi Vebø and Dr. scient Ågot Aakra)
Washing/Hybridization Station HS 400 Pro

DNA microarray related projects
1. “Development of DNA microarray technology for functional genomics related to food-production and food quality.” 2001-2006 Funded by the Norwegian Research Council.
2. “Molecular Microbiology of Gram-positive food bacteria: Functional Genomics and Application.” 2004-2008. Funded by the Norwegian Research Council.
3. “DNA microarray based comparative genome hybridization (CGH) of Staphylococcus aureus.” 2006-2009.Univerity (UMB) financed PhD project.
4. “Approaches to Control multi-resistant Enterococci: Studies on molecular ecology, horizontal gene transfer, fitness and prevention.“ EU funded project. 2006-2009.

Bacterial Genome-wide microarrays used by LMG
Enterococcus faecalis V583 PCR-based
Enterococcus faecalis V583 oligo-based
Enterococcus faecium DO PCR-based
Staphylococcus aureus Oligo-based from TIGR
Staphylococcus aureus Affimetrix technology
Lactobacillus sake 23K Oligo-based

Updated: 24.08.07
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P.O. Box 5003
NO-1432 Aas
Norway

Phone: +47 64 96 59 00
Fax: +47 64 96 59 01
E-mail : ikbm@umb.no