Master Students - Molecular Cell Biology

Linn Wilhelmsen

Current and former master students in our research group:

Lene Sveen

Lene Sveen Foto: Lene Sveen

Lene studied the expression and activation of Protease Activated Receptors (PARs) in colon cancer cell lines. PARs are G-coupled receptors activated by serine proteinases such as thrombin, trypsin, elastase or cathepsin G. The effects of PARs are mediated through activated signal pathways, leading to transcription of genes involved in inflammation responses. PARs play an important role in both protection of and disease of the GI.
Lene showed that mRNA for all 4 PAR receptors are expressed in different types of intestinal epithelial cell lines. She also showed that the activating peptides (AP) AP1 and AP2 activated the intracellular signaling molecules ERK and JNK, while AP4 increased the level of cAMP. Similar experiments were done using a combination of stimulants, peptidases and intestinal juice.

Lene is currently working at Teach First

Kaia Mobråten

Kaia Mobråten Foto: Siri Eikrem Skotland

Kaia works with EP receptors in CaCa-2 cells. There are four different EP receptors, named EP1-EP4, and they all bind prostaglandin E2. Kaia looks at different signalling pathways that are activated when the CaCo-2 cells are stimulated with prostaglandin E2. Kaia started a Ph.D. supervised by Trude Wiklund, Tor Lea and Yngve Stenstrøm at IKBM, UMB, and is still frequently working in our lab.

Louise Bjerkan (2008/2009)

Louise Bjerkan Foto: Siri Eikrem Skotland

Louise is working with dendritic cells (DCs) which are differentitated from monocytes isolated from blood. Different studies are performed on monocytes, immature and mature DCs, including the EP receptor expression and the activity of important cell signaling pathways such as the NF-kB pathway. In addition, a co - culture system consisting of Caco2 cells, DCs and bacteria will be studied, where the intention is to probe the molecular communication between these cells. The goal is to aquire knowledge about how DCs influence the gene expression in Caco2 cells when DCs are stimulated with different types of bacteria.
Techniques employed: gradientcentrifugation and antigen coupled magnetic beads to obtain monocytes from blood, Real Time PCR for the study of EP receptor expression, Western Blotting to examine NF-kB activation,multiplex analysis for cytokine studies and micro-matrix studies to compare patterns of gene expression in Caco2 cells.

Louise is now in a Ph.D-programme at the Faculty of Dentistry, University of Oslo.

Torunn Sletten (2008/2009)

Torunn Sletten Foto: Siri Eikrem Skotland

Polyunsaturated fatty acids and epithelial cell function.

Torunn is investigating how the omega-3 and omega-6 fatty acids affect the epithelial cell function in Caco-2 cells. She will study the lipid composition in the plasma membrane, and the effects of proteins dependent on the specific lipid composition in plasma membranes, for example tight junctions.
To do her study she will use different techniques; Western blot, Gas chromatography- mass spectrometry (GC-MS), and transmission electron microscopy (TEM).

Torunn is going to start working at the Biochemistry Department, at the Institute for Cancer Research
http://www.rr-research.no/olsnes

Taslim Mirza (2008/2009)

Taslim Mirza Foto: Siri Eikrem Skotland

Taslim works with Trefoil peptide in HT-29 cells, and there are three types of trefoil peptides named TFF1, TFF2 and TFF3. Her main target will be TFF3 peptide. She will also work with Adenosine receptors in Caco-2, HT-29, T84 and Int 407. There are four different Adenosine receptores, named A1, A2a, A2b and A3, and they all are coupled to G-protein. She will use different techniques; RT-PCR and Western blot.

Mariann Haraldstad (2008/2009)

Mariann Haraldstad Foto: Siri Eikrem Skotland

In her master project Mariann studied the differentiation of Caco2 cells in co-culture with other cells. Much of her work concerned the development of protocols for the optimalization of techniques such as TEM, SEM, and immuno-labelling

Oppdatert: 13.10.11
Utskriftsvennlig versjon

Del med en venn:

Institutt for Kjemi, bioteknologi og matvitenskap

Institutt for kjemi, bioteknologi og matvitenskap, UMB
Postboks 5003
1432 Ås

Tlf.: 64 96 59 00
Faks: 64 96 59 01

E-post : ikbm@umb.no

Besøksadresse:
Bioteknologibygningen (nr. 44 på oversiktskartet)
Christian Magnus Falsens vei 1, 1432 Ås

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